MSc Student
Advisor: Edwin Kniha
1Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna
Coauthors:
David Sainitzer1, Betim Xhekaj2, Kurtesh Sherifi2, Julia Walochnik1, Edwin Kniha1
2Faculty of Agriculture and Veterinary, University of Prishtina “Hasan Prishtina”, Prishtina, Republic of Kosovo
Introduction: Tick-borne diseases pose an increasing threat to animal and human health, particularly in areas with frequent vector–host interactions. This study presents the first molecular investigation of tick-borne pathogens in ticks collected directly from companion animals during veterinary visits in Prishtina, Kosovo. Companion animals can act as sentinels for zoonotic transmission, providing valuable insights into local pathogen circulation.
Methods: During summer 2024, 304 ticks were collected from 120 dogs and 20 cats presented for routine veterinary care. Ticks were morphologically identified and subjected to DNA extraction for barcoding and PCR screening targeting Anaplasma, Borrelia, Piroplasmida, and Rickettsia. Barcoding results were compared with morphological identifications for quality control.
Results: Five different tick species were identified. Preliminary PCR screening detected Rickettsia DNA in several samples. Barcoding confirmed morphological identifications. Sequencing of positive samples and screening for other pathogens are ongoing.
Conclusion: Veterinary-clinic–based sampling is a practical, noninvasive approach for tick-borne pathogen surveillance. The detection of Rickettsia highlights the zoonotic potential of local tick populations and underscores the importance of preventive measures and awareness among veterinarians and pet owners. Continued analyses will clarify the diversity and public health significance of tick-borne pathogens in Kosovo.
